This work entails both imaging and therapeutic applications of biomedi-
cal optics. In both contexts, an understanding of light transport in tissues
is necessary. An overview of optical transport in tissues is given, includ-
ing scattering theory. Digital Fourier holographic microscopy is introduced
and applied as a means of metrology via optical scatter imaging. The later
portion of this work focuses on the clinical application of photodynamic
therapy of osteosarcoma. A 3D Monte Carlo model is developed as a pre-
dictive model of clinical applications of photodynamic therapy in treating
osteosarcoma within the medullary cavity of long bones. Prior to compiling
the Monte Carlo model, optical properties of bone tissues are determined
via diffuse reflectance spectroscopy.